pcr-cloning

James W. Demastes zodema at unix1.sncc.lsu.edu
Mon Feb 17 13:41:32 EST 1997


Has anyone cloned pcr products from mitochondrial cyt b?  I'm having
trouble with toxicity/recombination using DH5alpha.  If anyone has had
success, could you
let me know what E. coli strain and vector you used?  Many thanks -Jim


***************************
James W. Demastes
Museum of Natural Science
119 Foster Hall
Louisiana State University
Baton Rouge, LA  70803




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