What is the SMALLEST DNA BINDING DOMAIN?

Ashok Aiyar aiyar at ebv.oncology.wisc.edu
Sat Jan 11 16:24:21 EST 1997


On Sat, 11 Jan 1997 11:52:26 EST, jstrassw at OPAL.TUFTS.EDU <jstrassw at OPAL.TUFTS.EDU> wrote:

>Well, the Bovine Papillomavirus DNA binding domain (minimal) iosonlt 85 AA
>although tit forms a dimer.  It has the aadvantage of many characterized
>mutations, and an excellent crystal structure (1.2A) and  binds
>sequence-specific to a palindropme at 10 -12 M.
>
>I also study it!

The Kd value that you have listed for the minimal portion of "BPV-E2 
DNA binding domain" binding it's cognate site is very different from 
that which has been published.

For instance, from J. Virol. 71:828-831 (1997) (a publication from
the Androphy lab), the Kd of the 87 a.a minimal DNA binding domain 
for it's cognate site is listed as approximately 7.0 nM (i.e. 7 x 10e-9 M), 
while a larger fragment, containing 127 a.a has a Kd of about 0.9 nM, 
and even this is quite far from the picomolar affinity you have listed ....

Later,
Ashok
-- 
Ashok Aiyar, Ph.D.
McArdle Laboratory for Cancer Research
University of Wisconsin-Madison



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