Sponges

akd38 Holger.Stalz at uni-koeln.de
Tue Jul 7 10:08:06 EST 1998


I'm looking for a method of isolating  genomic DNA from the siliceous
sponge Geodia cydonium. I tried the QIAGEN Blood & Cell culture Kit but
wasn't succesful at all.
The main problem is caused by the silica pieces which I achieved after
producing single cells and the gemmulae which couldn't be lysed due to
the membrane associated silica pieces.
If anyone had ever heard of someone who had ever lysed any kind of
membranes of siliceous sponges, it would be nice if YOU could inform
me...






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