sequencing AT rich genes

Marcio Pie pie at bu.edu
Wed Aug 29 12:49:39 EST 2001


Hi all,

We've been trying to sequence around 1kb of ant mitochondrial DNA.
Although the PCR works fine, I'm having problems with the sequencing. We
can't get sequences larger than 500bp, and we suspect that it is because
of the very high AT content of the ant mtDNA. Has anyone had similar
problems?

I've been using an Applied Biosystems 377 automated DNA sequencer with XL
upgrade.

Thanks

Marcio

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Marcio R. Pie
Department of Biology
Boston University
5 Cummington St.
Boston, MA 02215

Phone: (617) 353-6974
FAX:   (617) 353-6340 
http://people.bu.edu/pie/


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