sequencing AT rich genes
pie at bu.edu
Wed Aug 29 12:49:39 EST 2001
We've been trying to sequence around 1kb of ant mitochondrial DNA.
Although the PCR works fine, I'm having problems with the sequencing. We
can't get sequences larger than 500bp, and we suspect that it is because
of the very high AT content of the ant mtDNA. Has anyone had similar
I've been using an Applied Biosystems 377 automated DNA sequencer with XL
Marcio R. Pie
Department of Biology
5 Cummington St.
Boston, MA 02215
Phone: (617) 353-6974
FAX: (617) 353-6340
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