How to build oligomers

[ZELJKOS at VMS.UCC.OKSTATE.EDU]

                        From: Zeljko Svedruzic 
                        Ph 405 744-9322
                        Fax 405 744-7799
                        E-mail zeljkos at vms.ucc.okstate.edu
              246 B Noble Research Center, Stillwatwater OK, 74078



======The problem description:=============================

          I do not know how to assemble oligomers of proteins that
 are given in the PDB files as monomers. As one example, porcine
 heart Lactate Dehydrogenase is a tetramer in the solution, and 
resolved crystal structure is a tetramer but only one monomer 
is entered in the PDB. The file name in the PDB is "pdb5ldh.ent".
How do I built a tetramer from that monomer? The cell size is
known, and the cell type is P32 2 1.
          I suspect there should be some matrix symmetry operation
that will just transform the coordinates. That is probably not 
something difficult to do since  a number of oligomeric protein
PDB files have coordinates of only one monomer. My address is 
given in the beginning of the letter.

With much appreciation 
Zeljko Svedruzic. March 5, 1996.