Sensitive alkaline phosphatase assay
TIY at poltorak.chem.msu.su
Sat Jun 20 11:11:53 EST 1998
>but the alkaline phosphatase activity is too low to be useful.
Dear Dudley Page,
Could you describe the experiment in more detail ?
For example, what APAse do you use (source, another data) ?
What is the expression system (E.coli) ? Do you experiment
with pH, temperature (APAse is periplasmic protein, therefore, it could be
easily regulated by changing 'outside' pH. E.coli Apase is relatively
thermostable- the molecule begins to dissociate into subunits with the activity
loss at 70-80 C) ? Moreover, E.coli APase activity strongly depends
on the MG and Zn ion concentrations -the ions are involved in active site
and molecule stabilization)
Some of that may help,
More information about the Molmodel