Dan Ebbole dje0282 at ZEUS.TAMU.EDU
Tue Dec 14 09:15:36 EST 1993

We have been using Interspex supplied Novozyme with good results.  However,
it seems to be very active: our normal protocol is for around 60 min
treatment of germinated N. crassa conidia for protoplasting.  This batch
gives complete protoplasting within 20 min, a 60 min digest yields
protoplasts with low transformability.
Dr. Daniel Ebbole
dje0282 at
Dept. of Plant Pathology and Microbiology
Texas A&M University
College Station, TX 77843-2132

More information about the Mycology mailing list