Novozyme

[Dan Ebbole]

We have been using Interspex supplied Novozyme with good results.  However,
it seems to be very active: our normal protocol is for around 60 min
treatment of germinated N. crassa conidia for protoplasting.  This batch
gives complete protoplasting within 20 min, a 60 min digest yields
protoplasts with low transformability.
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Dr. Daniel Ebbole
dje0282 at summa.tamu.edu
Dept. of Plant Pathology and Microbiology
Texas A&M University
College Station, TX 77843-2132