Not1 DIGESTS AND FIGE GELS.
PRADE at bscr.uga.edu
Thu Sep 23 18:25:49 EST 1993
In <27sj5p$990 at hobbes.cc.uga.edu> PRADE at bscr.uga.edu writes:
> How to make FIGE-GELS of Aspergillus nidulans Not1 digested
> chromosomal DNA ?
> Does anybody know why our DNA, prepared by protoplasting (NOVOZYM),
> embedding in agarose (SeaPlaque GTG), lysis in 1% sarcosil-protei-
> nase K (2 mgs/ml) and PMSF inactivation, does not leave the wells,
> even after Not1 or BamH1 digestions ?
> Thanks for any clues
> Prade at bscr.uga.edu
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