Not1 DIGESTS AND FIGE GELS.

ROLF PRADE PRADE at bscr.uga.edu
Thu Sep 23 18:25:49 EST 1993


In <27sj5p$990 at hobbes.cc.uga.edu> PRADE at bscr.uga.edu writes:

> 
> 	How to make FIGE-GELS of Aspergillus nidulans Not1 digested
> 	chromosomal DNA ?
> 
> 	Does anybody know why our DNA, prepared by protoplasting (NOVOZYM),
> 	embedding in agarose (SeaPlaque GTG), lysis in 1% sarcosil-protei-
> 	nase K (2 mgs/ml) and PMSF inactivation, does not leave the wells,
> 	even after Not1 or BamH1 digestions ?
> 
> 	Thanks for any clues
> 		Rolf
> 	Prade at bscr.uga.edu
> 



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