S.rolfsii mutagenesis

[Nancy Keller]

I'm looking for suggestions on best way to do mutagenesis w/ S. rolfsii. 
> This is a multinucleate, prob. heterothallic basidiomycete which produces
> no spores (of a useful quantity).  So, I thought to irradiate either
> mycelial fragments or protoplasts w/ UV.  Goal is to create some useful
> markers.  Any suggestions or experience w/ this fungus or similar ones? 
> Thanks, Nancy Keller, Texas A&M Univ., Plant Patholo & Microbiol