Peter J. Punt,MBL-TNO,Rijswijk,The Neth. PETER_PU at mbl-prog.mbl.TNO.NL
Wed Aug 31 06:41:11 EST 1994

Peter J. Punt, Anneke Kuyvenhoven and Cees A. M. J. J. van den Hondel

A mini-promoter lacZ gene fusion for the analysis of fungal transcription
control sequences 

Gene (in press) 1994

        A system for the in vivo analysis of fungal transcription control
sequences, based on a mini-promoter, was designed. The mini-promoter,
providing all sequences necessary and sufficient for transcription initiation,
was derived from the Aspergillus nidulans gpdA promoter region. Transcription
initiation was not affected by the introduction of transcription control
sequences directly upstream of the mini-promoter. Furthermore, the expression
of the mini-promoter was not affected by wide-domain carbon or nitrogen
control circuits. Using the mini-promoter vector, a previously identified
upstream activating sequence from the A. nidulans gpdA gene was further

Department of Molecular Genetics and Gene Technology, TNO Nutrition and Food
Research Institute, 2280 HV Rijswijk, The Netherlands

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