Heat resistant bacteria???
andrzej at UIDAHO.EDU
Tue Dec 27 13:18:08 EST 1994
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> From: stamets1 at aol.com (Stamets 1)
> Subject: heat-resistant bacteria fouling pure cultures of fungi
> Date: 24 Dec 1994 15:36:32 -0500
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> Reply-To: stamets1 at aol.com (Stamets 1)
> Fellow Mycologists
> On heat resistant bacteria fouling pure cultures:
> Just what could be a helpful note. Most of you making culture
> media for fungi autoclave for 30-45 minutes at 15 psi (1kg/cm-2).
> Most use wisely use filtered/purified water. I usually do not.
> With the massive rains we have had here in the
> past 3 weeks - the worst flooding in 75 years -
> the ground water has become
> unusually rich with sediments/nutrients,
> causing a bacterial bloom, an order of magnitude
> greater than that which I have seen in years.
> Running parallel trials, I was shocked to find
> that malt extract agar media "spontaneously"
> contaminated with bacteria after 2 1/2 hours of autoclaving.
> The contaminating agents appeared 3 weeks after pouring.
> So goes the concept of "sterilization".
> Clearly, the more contaminants at the front end of the
> process, the more which survive, post treatment.
> We have had to return to osmosis-filtered water,
> which, for a production facility is not without its limitations.
> A friend in Europe, Peter Oei, author of several books on
> mushroom cultivation, e-mailed me with what appears to be
> a similar problem. Collectively, we have more than 40 years
> of culture experience. This is the first time we both have
> seen this. I can only wonder - Could bacteria with greater
> heat tolerance be evolving? Is world-wide ground water contamination and
> nutrient flow creating a new environment for the evolution of
> these bacteria?
> How many physcians really comprehend what "sterilization"
> means. Most hospital disposal programs for destruction of pathogens
> would not compare favorably with my experiences.Do I sound alarmist?
> Are my concerns unjustified, unscientifically based. I invite your
> comments - especially from bacteriologists.
> On another matter, have any of you heard about
> "gene migration" of antibiotic-resistant properties of bacteria
> which have not been directly to antibiotics exposed?
> Evolution is a powerful force. I can only wonder at what
> unwitting participation humans, laboratories, hospitals, day
> care centers, and immunized populations play in the evolution
> of new generation pathogens.
> On that hopeful note, I remain humbled by the power of the
> invisible landscape.
> Happy New Year!
> paul stamets
It is very easy to draw conclusion like above. I suggest to check
performance of your autoclave by using internal mercury termometer. You
can by autoclave termometer from VWR.
Andrzej Paszczynski University of Idaho
Andrzej at crow.csrv.uidaho.edu
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