neurospora and bialaphos

Dan Ebbole dje0282 at SUMMA.TAMU.EDU
Tue Jul 5 09:27:55 EST 1994


We are interested in using bialaphos as a selectable marker for
transformation as described by Martin Pall in the Fungal Genetics
Newsletter Vol 40 p. 58.  It was found that nitrogen-free medium N
supplemented with proline was required for good growth inhibition.  What
other media have been used?  Do other amino acids  work as nitrogen
sources? 

Also can someone tell me the address of a good supplier of
bialaphos/basta/ignite?  Thanks.
------------------------
Dr. Daniel Ebbole
dje0282 at summa.tamu.edu
Dept. of Plant Pathology and Microbiology
Texas A&M University
College Station, TX 77843-2132




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