how to protoplast lichens?

[Scott Kroken]

Has anyone out there succeeded (or even attempted) to make protoplasts of
cultured lichen mycobiont?   I suspect this may be easier planned then
done (the culture is not fluffy filaments, but a tight little wad of
tissue with tough cell walls), so I would much appreciate ideas that you
may have.  Thanks.


______________________________________________________________________S
ott Kroken                             internet: 
kroken at nature.berkeley.edu
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