use of phleomycin in transformation
SCHURENF at biol.rug.nl
Tue Mar 29 08:22:52 EST 1994
I'm working with the basidiomycete Schizophyllum commune and we are
using the phleomycin (bleomycin) resistance system for transformation.
If we use 0.5 M MgSO4 as osmotic stabiliser during regeneration of the
protoplasts we obtain 1000-5000 times as many transformants compared
to using 1M sorbitol. This is not caused by differences in
regeneration efficiency and also not by obtaining false positives.
However, if we try the same procedure for other basidiomycetes we do
seem to obtain false positives. Who recognizes this from his/her own
work or has any idea how MgSo4 interacts with phleomycin?
Dept. Plant Biology
University of Groningen
e-mail: schurenf at biol.rug.nl
More information about the Mycology