Autonomous replication

Chuck Staben staben at
Thu Nov 17 10:03:43 EST 1994

I received a few replies concerning autonomous replication in Neurospora.
Most detailed was from fons.debets* who said that he had
compared transformation of N.c. with a hygromycin vector alone and in 
combination with an autonomous vector.  They observed NO increase in
transformation frequency in Nc, unlike the 10-100 fold increase they
observed with A. nidulans and some other fungi.  
This suggests that the Aspergillus element does not function in Nc,
any other direct experience?

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