protein expression

GARY HERMANSON ghcytel at cerfnet.com
Tue Nov 22 18:06:56 EST 1994


We harvest a clone of Aspergillus niger that secretes a particular enzyme
and have found that the type of growth media makes a huge effect.  We're
using industrial type rich medias and it's not obvious why with some we get
large yields and with  others we get nothing. I haven't done northerns yet
but my bet is on protein stability in the media. Does anyone have a good
reference that talks about media components?   



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