pcr from colonies
geomay at MOLBIO.CBS.UMN.EDU
Sun Oct 9 15:18:32 EST 1994
We use Lee's method (BioTechniques 15, Sept.1993). We start with 3 small squares of mycelium, each about 1-1.5 mm sq. and trim away agar underneath. These go into 0-ring Eppendorf tubes with 50 uL extraction buffer. We follow the method and then use 2
uL/25 uL reaction. It comes out about right for Coprinus cinereus, sometimes a 1:10 dilution works better. The mycelium is freshly grown and the squares are removed about 1 cm back from the colony edge.
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