PFGE separation of mt DNA?

[koenigg at rnisd0.DNET.roche.com]

Does anyone have a protocol for pulsed field electrophoretic separation of fungal mitochondrial DNA?

We are studying the chromosomal arrangements of the human pathogens Coccidioidesimmitis and Histoplasma capsulatum.

We would like to apply the right PFGE conditions to separate the mt DNA from
the chromosomes.

I'd appreciate any references.

Thank you.
Gina Koenig
Roche Molecular Systems
Koenigg at rnisd0.dnet.roche.com        (510)-814-2815  fax (510) 522-1285
1145 Atlantic Avenue
Alameda, CA  94501  USA