prerservation of pleurotus
michael-clark at uiowa.edu
Wed Dec 20 21:57:30 EST 1995
Cynthia Wang wrote:
>I am trying to preserve the mycelia of Pleurotus ostreatus. I tried to
>freeze the culture broth/suspension but the revival rate was very low
>and it takes 3 weeks to recover the culture from frozen to an agar
>culture. Any suggestions?
Did you use any cryoprotectant? The standard for yeast work is 10%
glycerol. You could also try 10% DMSO, which is what we use for
freezing mammalian cells. These agents dramatically improve survival
of frozen microorganisms. Also, what temperature are you freezing at?
The colder the better. -70C is the standard for long term
cryopreservation of bacteria and yeast. Survival is reduced at -20C.
BTW: slow freezing works better than flash freezing (i.e. immersion
into liquid N2).
Or it may be that Pleurotus just doesn't freeze well. I've successfully
frozen at -70C, with excellent recovery, both Coprinus comatus and
Stropharia ruguloso-annuata in 10% glycerol. But I'm still working to
freeze morel mycelium (any suggestions would be helpful, hint hint to
anyone in the know!)
--University of Iowa
--Molecular Biology Program
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