calcium in fungi

[Nora Plesofsky-Vig]

I would appreciate some help in designing/assessing experiments to  
determine cytoplasmic calcium levels in Neurospora crassa or another  
fungus. Specific questions are: 

	(1) Does the esterified fura-2 work in Neurospora for  
fluorescent measurements of calcium, or what are the drawbacks to its  
use in fungi? 

	(2) Does the calcium ionophore ionomycin work in Neurospora,  
or is there a better alternative?

Any handy literature references would also be appreciated.

Nora Plesofsky-Vig