calcium in fungi
npv at MANI.CBS.UMN.EDU
Tue Feb 7 15:54:08 EST 1995
I would appreciate some help in designing/assessing experiments to
determine cytoplasmic calcium levels in Neurospora crassa or another
fungus. Specific questions are:
(1) Does the esterified fura-2 work in Neurospora for
fluorescent measurements of calcium, or what are the drawbacks to its
use in fungi?
(2) Does the calcium ionophore ionomycin work in Neurospora,
or is there a better alternative?
Any handy literature references would also be appreciated.
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