How to break fungal mycelium
S.Leangon at massey.ac.nz
Thu Feb 9 01:51:49 EST 1995
I have 2 questions, any suggestions would be great appreciated.
1. Does anybody know the good procedure to break the fungal mycelium ?
I am working with Aspergillus niger. I grow it on the surface of a semi-
solid medium prepared from sweet potato root. The mycelium sheet of the
old culture is rather hard to break in the porcelain mortar.
2. I am trying to assay ATP, AMP, NAD and NADH in this fungus. Does
anyone ever experienced with this assays (especially in fungal mycelium).
At the moment I follow the procedures from "Bergmeyer Methods of Enzymatic
Analysis" but I am wondering if there are any modifications or cautions.
Please contact me at :
"S.Leangon at massey.ac.nz"
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