stewart at bud.peinet.pe.ca
Sat Feb 18 12:18:45 EST 1995
To the person attempting microwave media sterilization:
I asked for info on this on this newsgroup a couple of months ago, and
got a wide range of replies; everything from, "forget it, it won't work"
to, "our lab has never had contamination in years wih our microwaved
instruments". The general consensus seems to be that it is chancy at
best. I have had good luck with liquid media by nuking for 30 minutes on
High. You need to have lots of headroom in the bottle, since it will
boil over otherwise. I put a couple of coffee mugs full of water and that
reduces the loss of water from the media. You will still lose about
10-20%, so make the media up with extra water to allow for this.
My guess is you should presoak the grain, straw or woodchips, and
include a fair amount of water when you nuke it. I had to nuke only three
bottles of straw/grain at a time for about an hour. No contamination yet,
but I feel microwave works best on small volumes of liquid, where
hot/cold spots matter less due to mixing.
Maybe your inquiry will spark discussion on this. I feel the
topic is far from having reached a consensus. Wadda ya say experts?
......Paul from the Great White Northeast.
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