Do I have cell wall fraction of yeast

SMazzer smazzer at aol.com
Mon Feb 27 19:59:28 EST 1995


>It can be removed from the cells by 
>washing them in buffer before you break open the cells.  A little
>detergent 
>in the buffer (such as Triton X-100) can also help remove the mucilage.

Be careful using this method.  Triton X-100 (as well as other detergents)
tend to give false-positive results in many commonly-available protein
assays.  

Paula Mazzer ("PMazzer at AOL.com")



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