heat resistant bacteria

Paul T. Magee ptm at MOLBIO.CBS.UMN.EDU
Mon Jan 2 14:31:31 EST 1995

The phenomenon of bacteria surviving a standard autoclaving is neither new nor
unique.  What's happening is that the spores are either not getting to 120 or
there are so many that a few survive.  The best way to deal with this problem
is Tyndallization, which is to autoclave twice, with a couple of hours between
the two treatments.  Heat causes the spores to germinate, and then they become
susceptible to standard sterilization techniques.
Pete Magee

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