r3603207 at CC.NTU.EDU.TW
Thu Jul 6 11:08:35 EST 1995
Does anyone have experience about manipulation of monospore isolation?
How to do monospore isolation? Can anyone tell me?
I have culture of Geotricum sp.in malt extract agar slants, and after 3
days of incubation at 30 degree celsius, I used 1% triton solution to obtain
the spores. The spores was incubated in malt extract agar but the germination
rate was very poor. Did I do anything wrong in the procedure?
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