Fri Jul 7 17:00:07 EST 1995
In article <Pine.SUN.3.91.950706155910.1369A-100000 at ccsun10>,
r3603207 at CC.NTU.EDU.TW says...
> Does anyone have experience about manipulation of monospore isolation?
>How to do monospore isolation? Can anyone tell me?
> I have culture of Geotricum sp.in malt extract agar slants, and after
>days of incubation at 30 degree celsius, I used 1% triton solution to
>the spores. The spores was incubated in malt extract agar but the
>rate was very poor. Did I do anything wrong in the procedure?
I would suggest you try your isolation with a lower concentration of
triton. I have used 0.0001 % and have been sucessful with Aspergillus
Best of luck,
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