DNA from herbarium specimens
staben at pop.uky.edu
Mon Jul 10 07:12:34 EST 1995
John Taylor's lab has very wide experience with preparing DNA from many fungi,
although perhaps not dried specimen's. You can e-mail him:
jtaylor at violet.berkeley.edu.
I use a simple procedure like one that John published in which one simply incubates
dried mycelium in 50 mM Tris (pH=8), 50 mM EDTA, 2% SDS, 1% 2-mercaptoethanol, 100
microgram/ml proteinase K at 60 degrees overnight. Phenol extract 2X. CHCl3. Ethanol
precipitate. Can further purify on CsCl gradients for large preps. This also works for
my lab to do minipreps from conidia for PCR.
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