DNA from herbarium specimens

Chuck Staben staben at pop.uky.edu
Mon Jul 10 07:12:34 EST 1995


John Taylor's lab has very wide experience with preparing DNA from many fungi,
although perhaps not dried specimen's.  You can e-mail him:
jtaylor at violet.berkeley.edu.
I use a simple procedure like one that John published in which one simply incubates
dried mycelium in 50 mM Tris (pH=8), 50 mM EDTA, 2% SDS, 1% 2-mercaptoethanol, 100 
microgram/ml proteinase K at 60 degrees overnight.  Phenol extract 2X.  CHCl3. Ethanol
precipitate.  Can further purify on CsCl gradients for large preps.  This also works for
my lab to do minipreps from conidia for PCR.




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