In vitro mutagenesis

Gregory Staurt May gsmay at bcm.tmc.edu
Thu Jun 15 11:10:20 EST 1995


I have a general question for the community regarding methods for in 
vitro mutagenesis.  We would like to make conditional lethal mutations
in a gene we have cloned.  We know the gene is essential so it should be 
possible to make such mutations.  What I would like is a simple random 
mutagenesis method that has been demonstrated by others that works.  Any 
and all suggestions are welcome.  I have thought of using Stratagene's 
XL1-Red strain for this.

Thanks,
Greg




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