Neurospora crassa PCR

Veronica Wolf wolfv at ESSEX.HSC.COLORADO.EDU
Fri Jun 30 12:21:09 EST 1995


I am having a difficult time PCRing N. crassa and I was wondering if 
anyone could direct me to a paper that could guide me on optimal 
conditions i.e. buffer, template and primer concentrations, enzyme 
amount, temperatures and cycle number.



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