mito membrane protein fractions
Nora Plesofsky-Vig
npv at MANI.CBS.UMN.EDU
Thu Mar 2 17:51:35 EST 1995
I have not actually subfractionated mitochondria, but here are some
references for the published procedures:
Sottocasa GL et al., J. Cell Biol. 32 (1967):415-436
Riezman H et al., EMBO J. 2 (1983): 1105-1111
Freitag H et al., Eur. J. Biochem: 126 (1982): 197-202
" " : 123 (1982): 629-636
The protein content of the inner mitochondrial membrane is apparently
much greater than that of the outer membrane. You could make
submitochondrial particles (by sonication) to separate the membranes
from the matrix proteins.
Nora Plesofsky-Vig
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