Fungal Genetics Conference at Asilomar

Kevin McCluskey mccluske at ag
Mon Mar 6 19:39:27 EST 1995

(I am posting this for Hans VanEtten.  If anyone needs to contact
his lab before the 13th, please contact me by E-mail.
Kevin McCluskey)

To individuals that will be attending the 18th Fungal 
Genetics Meeting at Asilomar on March 21 to 26:  
Below is the schedule for the workshop on "Pathogenicity 
and avirulence genes of plant pathogens:  new and emerging
techniques for their identification and isolation" which 
will take place on the afternoon of March 22.  The objective 
of this workshop is to highlight approaches that allow the 
isolation of pathogenicity or avirulence genes without prior 
knowledge of the gene's biochemical function or activity.
Some of the presenters will supply hand-outs of the 
techniques they will discuss.  There is still some room 
on the program during the "general discussion periods" 
for additional presentations of approaches that are not
listed on the program as well as time for others to share 
their experience with the listed techniques.  If you have  
experience with a technique that has allowed you to isolate a 
pathogenicity or avirulence gene or experience with the 
listed techniques and would be willing to share that information,
please contact me (Hans VanEtten) 

        Unfortunately, because of my travel plans, I will not 
be accessing my email until March 13.  However, hopefully communications 
initiated at that date or shortly there after will still be in time to 
make arrangements for participation in the workshop.

Workshop on "Pathogenicity and Avirulence genes of 
Plant Pathogens:  New and Emerging Techniques for their 
Identification and Isolation."

1:30    Introduction - Hans VanEtten, University of Arizona.
1:35    "Introduction of the restriction enzyme-
            mediated integration (REMI) technique and experience 
            with Magnaporthe grisea" - Jim Sweigard, DuPont.
1:55    "REMI-induced mutations in several toxin-
           producing fungi." - Olen Yoder, Cornell University.
2:10    Open to discussion on the REMI technique.
2:25    Transposons:  "Toward the development of 
           a gene-tagging system in Fusarium oxysporum" 
           - Marie Josee Daboussi, Universite Paris Sud.
2:45    Open floor on use of transposons.
3:00    "Differential cDNA screening strategies for the 
            identification of stage-specific gene expression 
            in pathogenic fungi." - Nick Talbot,
            University of Exeter.
3:15    "Identification of pathogenicity genes in U. maydis 
             by differential display" - Regina Kahmann, 
             University of Munich.
3:30    "Identification of virulence genes by negative selection."
             - David Holden, Hammersmith Hospital, London.
3:45    Open to floor on approaches to isolate pathogenicity 
           or avirulence genes by first isolating or identifying 
           their transcripts or promoters.
4:00    Break.
4:15    "Determining if pathogenicity genes are on dispensable
             chromosomes." - Hans VanEtten, Univeristy of Arizona.
4:30    "Telomere-mediated chromosome breakage and analysis of
            pathogenicity determinants on dispensable chromosomes."
             - Corby Kistler, University of Florida.
4:45    Open to floor on techniques that take advantage of 
            pathogenicity or avirulence genes being on dispensable 
             genomic DNA.
5:00    "Mapping genes in Phytophthora infestans using bulked 
            segregant analysis." - Howard Judelson, 
            University of California, Riverside.
5:15    "Genetic and physical mapping with amplified 
            fragment length polymorphisms (AFLPs) and 
            bacterial artificial chromosomes (BACs)." -
             Richard Michelmore, University of California, Davis.
5:30    Open to floor for discussion of physical mapping 
           strategies and general discussion.

**                                                                      **
**  Kevin McCluskey                       Department of Plant Pathology **
**                                        Forbes Building Room 104      **
**  Phone: (602) 621-9390                 University of Arizona         **
**  FAX: (602) 621-9290                   Tucson, AZ 85721              **
**                                                                      **

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