Neurospora RNA for RNase protection

rlab davis rdlab at
Sun Mar 12 19:14:03 EST 1995

Is anyone using a particular RNA isolation protocol for Neurospora which 
yields total RNA suitable for RNase protection?  I seem to be having trouble 
with DNA contamination using techniques published in the FGN.

Ideally, I would like a technique which is quick, easy and doesn't involve 
isolating mRNA from total.  Is this wishful thinking?

If you have any suggestions, please email me.  Thanks in advance.

Martin Hoyt 
Dept. of Molecular Biology and Biochemistry, Univ. Calif. Irvine
mahoyt at

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