HELP: Fungal nuclei isolation

Brett Tyler bmtyler at UCDAVIS.EDU
Thu Mar 30 12:46:23 EST 1995


Dear Jay,

the reference for an excellent method of nuclei isolation from Neurospora
is "Baum, J.A. and Giles, N.H. (1985)
J. Mol. Biol. 182, 79-89."

>I have done some literature searching, however finding
>publications on the method I need has proven difficult.
>If anyone out there is familiar with techinques for
>isolation of nuclei from filamentous fungi, I would
>appreciate any help you would care to offer.
>     I would also be interested in any information regarding
>techniques for subcellular fractionation of filamentous fungal
>cultures.  What is a good (clean) method for subcellular
>fractionation, and how was it determined which fraction contains
>what cellular components?
>
>Thanks
>Jay Phillips
>JPhillips at Genencor.com


****************
Brett Tyler
Professor
Department of Plant Pathology
University of California, Davis
bmtyler at ucdavis.edu
****************





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