HELP: Fungal nuclei isolation

Jay Phillips JPhillips at Genencor.com
Mon Mar 27 20:04:24 EST 1995


I have done some literature searching, however finding 
publications on the method I need has proven difficult.
If anyone out there is familiar with techinques for 
isolation of nuclei from filamentous fungi, I would
appreciate any help you would care to offer.
     I would also be interested in any information regarding
techniques for subcellular fractionation of filamentous fungal
cultures.  What is a good (clean) method for subcellular 
fractionation, and how was it determined which fraction contains
what cellular components?

Thanks
Jay Phillips
JPhillips at Genencor.com



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