Plasmid Preparation for transformation.
mb1gt at sunc.sheffield.ac.uk
Fri May 19 11:04:45 EST 1995
Gregory May (gsmay at bcm.tmc.edu) wrote:
: I have a rather specific question for those transforming Aspergillus nidulans.
: My group has tried several methods of preparing plasmid DNA for transformation
: but the most reliable one still is to purify the DNA on CsCl ethidium bromide
: gradients. This is tedious when you want to make multiple constructs and test
: them for function etc. Does anyone have a reproducible method of making
: plasmid DNA that transforms each and every time?
Trying Quiagen ourselves at present.
This is a very important point, though. In searching for
genes amongst a small group of cosmid clones (e.g. from
FGSC library) this is a problem.
Hope you get more constructive answers!
University of Sheffield
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and that's yer own, yer own, yer own" - The Levellers
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