Favorite DNA isolation protocol

Chris Skory skorycd at ncaur1.ncaur.gov
Tue Nov 7 08:39:53 EST 1995

I apologize if this topic has shown up before on this news group. 
But, I was wondering what methods fellow molecular biologist 
working with filamentous fungi prefer for isolating genomic DNA 
from fungi.  I've tried numerous methods and am not very content 
with any particular one.  Do most of you use CsCl or CTAB for 
getting rid of carbohydrate?  The best luck I have had with 
isolating high quality genomic DNA has been with grinding in 
liquid nitrogen (with mortar and pestle), extracting a few times 
with phenol/chlor, and finally separating with CsCl.  
Unfortunately, this procedure is very time consuming.  Any other 

Christopher D. Skory, Ph.D. 		 
Research Microbiologist

National Center for Agricultural	        
  Utilization Research, USDA-ARS 	             
Fermentation Biochemistry Unit 
Peoria, Illinois 61571-3902       

E-mail:  skorycd at ncaur1.ncaur.gov

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