disruption of fungal cells

[Nora Plesofsky-Vig]

To disrupt Neurospora cells, we usually use a heavy duty Braun MSK  
homogenizer, in which the cells (in buffer) are forcefully bombarded  
with 1 mm glass beads. However, we have also successfully extracted  
small quantities of cells by vortexing them with 0.1 mm glass beads  
and buffer; the smaller glass beads worked much better than the  
larger under these conditions. About 1 ml of fungal culture was  
collected and disupted in an Eppendorf tube with 100 mg of the glass  
beads. This might be a place to start for extracting cytosolic  
proteins. Good luck.

Nora Plesofsky-Vig
nora at biosci.cbs.umn.edu