disruption of fungal cells

Nora Plesofsky-Vig npv at MANI.CBS.UMN.EDU
Thu Oct 26 18:29:14 EST 1995


To disrupt Neurospora cells, we usually use a heavy duty Braun MSK  
homogenizer, in which the cells (in buffer) are forcefully bombarded  
with 1 mm glass beads. However, we have also successfully extracted  
small quantities of cells by vortexing them with 0.1 mm glass beads  
and buffer; the smaller glass beads worked much better than the  
larger under these conditions. About 1 ml of fungal culture was  
collected and disupted in an Eppendorf tube with 100 mg of the glass  
beads. This might be a place to start for extracting cytosolic  
proteins. Good luck.

Nora Plesofsky-Vig
nora at biosci.cbs.umn.edu



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