Need info on LactoPhenol Blue
rlejeune at vnet3.vub.ac.be
Mon Sep 11 09:11:15 EST 1995
I would like any info, references, pointers,... on Lactophenol Blue (Merck
cat Nbr 13741).
What is the dye used in this solution? (The dye is identified as C.I.
42780 on the bottle).
Is it Trypan Blue? (I have a paper by Rhigelato et al. (1968), J. Gen.
Microb. 50:399 in which staining of filamentous fungi with 'trypan blue in
lactophenol' is described).
What part of the cells gets stained, and what makes it specific for
Is the staining intensity dependent on the physiological state of the cell
(before it was fixed)?
Dep. Chem. Eng. - Free Univ. Brussels
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