mushvg at ns.via.nl
mushvg at ns.via.nl
Fri Sep 15 13:48:24 EST 1995
In article <950912172459_97679946 at mail04.mail.aol.com>, <MZieg1234 at aol.com>
> From: MZieg1234 at aol.com
> Newsgroups: bionet.mycology
> Subject: Chanterelle?
> Date: 12 Sep 1995 20:24:20 -0700
> Organization: BIOSCI International Newsgroups for Molecular Biology
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> Sender: daemon at net.bio.net
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> I am trying to put Chanterelles into culture and have a question concerning
> how to identify the mycelium. I took some central tissue from a young
> Cantharellus cibarius and plated it into MEA agar containing amoxicillin.
> Bacterial growth was evident and probably due to the resident bacteria. On
> several plates a slow growing mycellium grew out from the zone of bacteria.
> It looks like many other I have in culture and not like a mold. White,
> branching, non-aerial. I transfered this non-bacterial zone to DCR media (a
> plant tissue culture media designed for conifers) as I thought it would help
> mimic in vivo conditions. Indeed growth is somewhat faster and thicker.
> Anybody have Chanterelle in culture who could suggest a way to i.d. it?
> Thanks Mike Ziegler.
Approximately 10 years ago Gerben Straatsma, then a Ph.D student, carried out a
number of studies on the cultivation of C.cibarius. Strain collection and
identification were published as Straatsma, G., Konings, R.N.H. & Van Griensven
L.J.L.D. (1985) A strain collection of the mycorrhizal mushroom C.cibarius
obtained by germination of spores and culture of fruit body tissue. Trans. Br.
Mycol. Soc. 85, 689-697. Later it was found that C. grows best at high CO2
concentratio. See: Straatsma, G. & Van Griensven, L.J.L.D.(1986) Growth
requirements of mycelial cultures of the mycorrhizal mushroom Cantharellus
cibarius Fr. Trans. Br. Mycol.Soc.87,135-141.Later the work was extended by
Lynn Moore: Moore, L.M., Jansen,A.E. & Van Griensven, L.J.L.D. (1990) Pure
culture synthesis of ectomycorrhizas with Cantharellus cibarius. Acta Bot.
Neerland. 38, 273-278. The latter was really promising but continuation was not
Hope you may be able to use this.
Leo J. Van Griensven.
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