Chanterelle?

[mushvg at ns.via.nl]

In article <950912172459_97679946 at mail04.mail.aol.com>, <MZieg1234 at aol.com> 
writes:
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> From: MZieg1234 at aol.com
> Newsgroups: bionet.mycology
> Subject: Chanterelle?
> Date: 12 Sep 1995 20:24:20 -0700
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> I am trying to put Chanterelles into culture and have a question concerning
> how to identify the mycelium. I took some central tissue from a young
> Cantharellus cibarius and plated it into MEA agar containing amoxicillin.
> Bacterial growth was evident and probably due to the resident bacteria. On
> several plates a slow growing mycellium grew out from the zone of bacteria.
> It looks like many other I have in culture and not like a mold. White,
> branching, non-aerial. I transfered this non-bacterial zone to DCR media (a
> plant tissue culture media designed for conifers) as I thought it would help
> mimic in vivo conditions. Indeed growth is somewhat faster and thicker.
>  Anybody have Chanterelle in culture who could suggest a way to i.d. it?
>  Thanks Mike Ziegler.

Approximately 10 years ago Gerben Straatsma, then a Ph.D student, carried out a 
number of studies on the cultivation of C.cibarius. Strain collection and 
identification were published as Straatsma, G., Konings, R.N.H. & Van Griensven 
L.J.L.D. (1985) A strain collection of the mycorrhizal mushroom C.cibarius 
obtained by germination of spores and culture of fruit body tissue. Trans. Br. 
Mycol. Soc. 85, 689-697. Later it was found that C. grows best at high CO2 
concentratio. See: Straatsma, G. & Van Griensven, L.J.L.D.(1986) Growth 
requirements of mycelial cultures of the mycorrhizal mushroom Cantharellus 
cibarius Fr. Trans. Br. Mycol.Soc.87,135-141.Later the work was extended by 
Lynn Moore: Moore, L.M., Jansen,A.E. & Van Griensven, L.J.L.D. (1990) Pure 
culture synthesis of ectomycorrhizas with Cantharellus cibarius. Acta Bot. 
Neerland. 38, 273-278. The latter was really promising but continuation was not 
funded.
Hope you may be able to use this.
Leo J. Van Griensven.