Flourescent fading GFP
claudes at essex.hsc.colorado.edu
Sat Aug 17 11:26:09 EST 1996
Has anyone ever observed GFP bleaching in certain organisms?
I am expressing GFP in a fungus and I have noticed that the GFP is
fading under extended UV exposure. This occurs very quickly when I put
on a microscope slide, which I presume is due to lack of oxygen.
However, it occurs more slowly when I leave the microscope slide off.
As far as I can tell, the bleaching is irreversible.
My guess is that the chromophore formation is slightly altered in this
fungus, and the result is a lower stability.
Dr. S. Canada
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