Aspergillus protein extracts

Javier Valdez jvaldez at DQBFCQ.UNCOR.EDU
Wed Aug 28 14:13:29 EST 1996


Dear Mycologists:

I am having problems trying to run SDS-PAGE with total Aspergillus nidulans
protein extracts. The gels have almost no proteins above 50 KD, and there is
a distortioned band at about 80 kd that seems to stop proteins from entering
the gels.We have tried several extraction conditions with no success so far.
I will welcome any suggestions or extraction protocols (that do not include
a bead beater).
Thank you very much in advance.

Javier E. Valdez
E-Mail: JValdez at dqbfcq.uncor.edu




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