Question about sectoring
MCSpear at msn.com
Sun Dec 22 03:39:19 EST 1996
Most broadly, sectoring is any change in the morphology of a fungal
culture. It is most classically exemplified by a wedge shaped area
of aberrent growth seen on a petri dish. In three dimensional
cultures like sawdust blocks, spawn bags, flasks, or compost beds it
can be a sphereical or hemisphereical area differing in aspect from
the surrounding mycelium.
The most common type of sector in the basidiomycetes is thick fluffy
to leathery growth. The most severe cases look like mushroom tissue
forming down in the substrate without any obvious organogenisis.
Sectoring is commercially undesirable and in extreme cases can be
evidence of instability or degeneration of the cell line. Most
experienced workers deliberately use techniques, media, and
conditions that tend to minimize sector formation. It is one of the
areas of art in culture maintenance.
Sectoring can also represents a source of genetic variation without
having to go thru meiosis.
There has been some recent work done by Paul Horgen at Erindale you
might want to look up.
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