Medium for sporulation?

K N and P J Harris ecoli at cix.compulink.co.uk
Thu Jan 4 14:10:36 EST 1996


Hello,
There is a distinct shortage of decent agar these days. PDA may start 
off fairly acidic and certainly will not take too many runs through the 
autoclave. Check preparation, if the slants were made from bulk that had 
been "cooked up" a few times it almost certainly won't set.

Freshly prepared slants shouldn't give problems.

Peter,
Soil Science,
Reading Uni, UK.




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