Medium for sporulation?
K N and P J Harris
ecoli at cix.compulink.co.uk
Thu Jan 4 14:10:36 EST 1996
There is a distinct shortage of decent agar these days. PDA may start
off fairly acidic and certainly will not take too many runs through the
autoclave. Check preparation, if the slants were made from bulk that had
been "cooked up" a few times it almost certainly won't set.
Freshly prepared slants shouldn't give problems.
Reading Uni, UK.
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