Medium for sporulation?

[K N and P J Harris]

Hello,
There is a distinct shortage of decent agar these days. PDA may start 
off fairly acidic and certainly will not take too many runs through the 
autoclave. Check preparation, if the slants were made from bulk that had 
been "cooked up" a few times it almost certainly won't set.

Freshly prepared slants shouldn't give problems.

Peter,
Soil Science,
Reading Uni, UK.