homologous recombination

[John Pitkin]

 An example of consistent ectopic gene integration in a system that
normally integrates foreign DNA homolgously is when the null phenotype of
the gene being disrupted is lethal.  I don't know what the gene is that you
are trying to disrupt, but could this be a possibility?  Is integration of
exogenous DNA into Septoria normally at homologous loci?   If not, then
longer integrating fragments may be useful, as is the case with Neurospora.


John Pitkin
MSU-DOE Plant Research Laboratory
MIchigan State University