Hi all out there,
I am planning to do an experiment to evaluate the growth of some
pathogenic fungal strains in a pH gradient with solid standard media.
Two commercial media will be used (CMA and PDA). I need information
about how to modify the pH of the Petri dishes to obtain a range
between 3.5 and 8.5. I understand that pH has to be adjusted by a
buffer, to prevent pH changes due to fungal growth. Here are some of
the questions I have to resolve:
- Which is the more suitable buffer to use?.
- At which concentration could have this buffer undesirable effects on
the fungal growth?.
- Which are the different amounts of each buffer constituent to be
added to the plates?.
- Any reference will also be appreciated.
E-mail me direct or post here, please.
Thank you very much in advance
Dep. Patologia Vegetal
Crtra. Cabrils s/n, E-08348 Cabrils, Barcelona, Spain