MALASSEZIA FURFUR

[S. Kesavan]

Hi there,

Is there anybody reading this who could give me some info on the lipid 
content of M. furfur cell wall (approx. 15% I believe....?)

Also, does anybody have a protocol as to how to de-lipidise yeast cell walls 
- can we use chloroform: ethanol 2:1 ? Are there any subsequent 
biochemical tests to find out if we have removed all the lipid?

Any help will be appreciated.

Thanks

Mr Shaleel Kesavan (2nd yr PhD- IMMUNOLOGY)