IGS pcr problem
moulliard at mbla.ucl.ac.be
Tue Mar 5 03:28:14 EST 1996
Could I have your opinion on this problem. I try to amplify the IGS
region of Penicillium terverticillate using the above primers
(CNS1/CNL12 or 28S-IGS/CNS1).
GAGACAAGCATATGACTAC CNS1 (beginnning of the 28S)
CTGAACGCCTCTAAGTCAG CLN12 (end of the 28S)
GTAAGCGGTCAAGTAGCCTT 28S-IGS cfr séquences (Accession number U27453
et Z27114 Genbank) (idem)
Unfortunately, the primers doesn't fail to amplify the IGS. What is
incredible, is that the primer 28S-IGS is present in the sequences of
the Penicillium hordei and the Aspergillus nidulans. With the Fusarium
oxysporum, the region is amplify with the two couple of primers.
Have you an idea about the problem?
Charles Moulliard (moulliard at mbla.ucl.ac.be)
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