VAM (AMF) staining.
Stephen P. Bentivenga
invam at WVNVM.WVNET.EDU
Fri May 3 15:34:37 EST 1996
On Fri, 3 May 1996 19:11:31 GMT ecoli at cix.compulink.co.uk ("K N and P J
>Force of circumstance made me carry out some standard staining of onion
>roots for VAM which had been dried and kept very dry for six months. So
>dry that they were very brittle. A normal KOH then HCl then Trypan Blue
>stain was applied. I am sure that the results were better than with
>fresh root material. Has anyone else had the same experience ?
>Department of Soil Science (The only one left in UK)
>University of Reading.
I routinely stain roots after they have been oven dried. I have found it is
the most convenient way to get both dry mass and % colonization data. It
works well with most plant species. I would not say that the results are
"better" but they are not any worse. The only problem I have found is that
the hyphae and arbuscules often collapse after drying, making good
photographs difficult. If you need pictures of the text book arbuscules I
don't recommend drying.
- Steve Bentivenga
.| Stephen P. Bentivenga, Ph.D. __ __
|| Division of Plant and Soil Sciences \ /
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