lysing of hypha

[David Hagerberg]

Hello!

I'm going to purify DNA out of hyphi from an extremely troublesome 
fungus, Drechmeria coniospora. Nothing seems to work. Now, I want to 
degrade the hyphal cell walls before lysing the hyphi. Do anyone have a 
protocoll for cell wall degradation that leaves the hyphi intact or is it 
better to lyse the hyphi at same time as the cell wall?

Best Regards