lysing of hypha
Wrightc2 at pilot.msu.edu
Thu Apr 17 18:46:13 EST 1997
If you are going for a DNA extraction from a pure culture, why don't you
lyophilize and grind the hyphae in liquid nitrogen? There are numerous
protocols for DNA extraction which will take it from there.
David Hagerberg <mik_daha at luecology.ecol.lu.sexx> wrote:
>I'm going to purify DNA out of hyphi from an extremely troublesome
>fungus, Drechmeria coniospora. Nothing seems to work. Now, I want to
>degrade the hyphal cell walls before lysing the hyphi. Do anyone have a
>protocoll for cell wall degradation that leaves the hyphi intact or is it
>better to lyse the hyphi at same time as the cell wall?
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