lysing of hypha

Chris Wright Wrightc2 at pilot.msu.edu
Thu Apr 17 18:46:13 EST 1997


If you are going for a DNA extraction from a pure culture, why don't you 
lyophilize and grind the hyphae in liquid nitrogen?  There are numerous 
protocols for DNA extraction which will take it from there.

                      Good luck,
                                    Chris  



David Hagerberg <mik_daha at luecology.ecol.lu.sexx> wrote:
>Hello!
>
>I'm going to purify DNA out of hyphi from an extremely troublesome 
>fungus, Drechmeria coniospora. Nothing seems to work. Now, I want to 
>degrade the hyphal cell walls before lysing the hyphi. Do anyone have a 
>protocoll for cell wall degradation that leaves the hyphi intact or is it 
>better to lyse the hyphi at same time as the cell wall?
>
>Best Regards





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